Research Article

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11/22/2013
Cloning; Expression; Major histocompatibility complex; Stone flounder

Major histocompatibility complex (MHC) class II genes play important recognition roles in the immune system in vertebrates. We cloned the MHC class II genes A and B in the stone flounder (Kareius bicoloratus). The full-length cDNA and DNA sequences of both genes were obtained, and their characteristic motifs were analyzed. The DNA sequence of stone flounder MHC ... more

J. Jiang; C. Li; Q. Zhang; X. Wang
02/27/2013
Chicken; Cloning; Expression; Nramp1 gene; Nramp2 gene; Polymorphism

Natural resistance-associated macrophage protein 1 and 2 encoding genes (Nramp1 and Nramp2) are related to many diseases. We cloned the cDNA of chicken Nramp1 and Nramp2 genes, characterized their expression and polymorphisms, and investigated the association of some SNPs with resistance to salmonellosis. The Nramp1 cDNA was 1746 bp long and the ... more

X.M. He; M.X. Fang; Z.T. Zhang; Y.S. Hu; X.Z. Jia; D.L. He; S.D. Liang; Q.H. Nie; X.Q. Zhang
12/18/2014
Bioinformatic analysis; Calcium-dependent protein kinases; Cloning; TgCDPK6; Toxoplasma gondii

Toxoplasma gondii is recognized as an opportunistic human pathogen with a worldwide distribution. Development of effective vaccines is considered the only ideal way to control T. gondii infection. However, only one live vaccine is commercially available for use in sheep and goats. Therefore, the identification of more effective antigenic proteins is very ... more

N.Z. Zhang; S.Y. Huang; D.H. Zhou; Y. Xu; J.J. He; X.Q. Zhu
09/02/2004
Cysteine proteinase; Expression; Heterodera glycines; Inhibitory activity; Nematode; Pro domain peptide

Cysteine proteinases (CPs) are synthesized as zymogens and converted to mature proteinase forms by proteolytic cleavage and release of their pro domain peptides. A cDNA encoding a papain-like CP, called hgcp-Iv, was isolated from a Heterodera glycines J2 cDNA library, expressed and utilized to assess the ability of its propeptide to inhibit proteinase in its active ... more

F.B. Silva; J.A.N. Batista; B.M. Marra; R.R. Fragoso; A.Carolina S. Monteiro; E.L.Z. Figueira; M.Fátima Grossi-de-Sá
12/08/2011
Alternative splicing variant; DRD2 gene; Expression; Pig; Polymorphism

The dopamine D2 receptor (DRD2) is a crucial mediator for normal physiological processes. We cloned the pig DRD2 gene, investigated its distribution in tissues and identified polymorphisms by RT-PCR, quantitative real-time PCR and direct sequencing. Two Yorkshire pigs from Guangdong Academy of Agricultural Sciences (Guangzhou, China) were selected to clone the gene and ... more

H.P. Xu; X.M. He; M.X. Fang; Y.S. Hu; X.Z. Jia; Q.H. Nie; X.Q. Zhang
02/28/2013
Adipocytokines; Expression; Insulin resistance; Polycystic ovary syndrome; Subcutaneous adipose tissue

We reviewed emerging evidence linking serum levels and adipose tissue expression of leptin and adiponectin in women with polycystic ovary syndrome (PCOS). Previous data obtained by our group from a sample of overweight/obese PCOS women and a control sample of normal weight controls, both stratified by BMI, were reanalyzed. Circulating levels of leptin and adiponectin were determined by ... more

S.B. Lecke; D.M. Morsch; P.M. Spritzer
09/12/2013
Expression; Heterosis; MRLC2 gene; Sequence analysis; Tianfu goat

To determine the molecular basis of heterosis in goats, fluorescence quantitative polymerase chain reaction (PCR) was performed to investigate myosin-regulatory light chain 2 (MRLC2) gene expression in the longissimus dorsi muscle tissues of the Tianfu goat and its parents, the Boer and Chengdu Ma goats. The goat MRLC2 gene was differentially expressed in the crossbreed ... more

H.G. Xu; G.Y. Xu; L. Wan; J. Ma
09/18/2013
Alcoholism; Atp5g3; Epigenetic; Expression; Mouse; Transcriptome

The mitochondrial ATP synthase, subunit c, isoform 3 gene (Atp5g3) encodes subunit 9, the subunit of the multisubunit enzyme that catalyzes ATP synthesis during oxidative phosphorylation in mitochondria. According to the Ensembl database, Atp5g3 in mice is located on chromosome 2 between 73746504 and 73749383 bp, within the genomic regions of two sets of quantitative ... more

Y. Huang; L. Wang; B. Bennett; R.W. Williams; Y.J. Wang; W.K. Gu; Y. Jiao
12/19/2012
Chitin deacetylase gene; Chitinase-3 gene; Expression; Hyriopsis cumingii; Potential functions

Chitin, the second most important natural polymer in the world, and its N-deacetylated derivative chitosan are found in a wide variety of organisms. These versatile biopolymers are associated with a broad range of biological functions. This article is the first to report the potential functions of 2 chitin metabolic enzyme genes from Hyriopsis cumingii. A chitinase-3 gene ( ... more

G.L. Wang; B. Xu; Z.Y. Bai; J.L. Li
12/21/2012
Expression; Heat shock protein 110; Heat shock protein 110 messenger RNA; Heat stress; In vitro; Primary myocardial cells of rat

We investigated and described the kinetics of heat shock protein (Hsp) 110 expression and distribution in rat primary myocardial cells exposed to heat stress in vitro. After incubation at 37°C for 72 h, myocardial cells were heat stressed at 42°C for 0, 10, 20, 40, 60, 120, 240, 360, and 480 min. Significant increases in aspartate transaminase, lactate dehydrogenase, and ... more

Z.J. Liu; Y.J. Lv; M. Zhang; Z.H. Yue; S. Tang; A. Islam; B. Rehana; E.D. Bao; J. Hartung

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