Research Article

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03/30/2003
DNA repair; Escherichia coli; Functional complementation

The bacteria Escherichia coli has been widely employed in studies of eukaryotic DNA repair genes. Several eukaryotic genes have been cloned by functional complementation of mutant lineages of E. coli. We examined the similarities and differences among bacterial and eukaryotic DNA repair systems. Based on these data, we examined tools used for gene cloning and ... more

L. Augusto-Pinto; C.Gustavo Re da Silva; Dde Oliveir Lopes; A. Machado-Silva; C.Renato Machado
10/04/2007
Co-regulation; Escherichia coli; Gene expression; Microarray analysis; Partial correlation; Transcriptional regulation

Transcriptional control is an essential regulatory mechanism employed by bacteria. Much about transcriptional regulation remains to be discovered, even for the most widely studied bacterium, Escherichia coli. In the present study, we made a genome-wide low-order partial correlation analysis of E. coli microarray data with the purpose of recovering regulatory ... more

D.F.T. Veiga; F.F.R. Vicente; M. Grivet; A. de la Fuente; A.T.R. Vasconcelos
05/25/2007
Adhesion; Avian; Escherichia coli; Plasmids

A 43-MDa conjugative plasmid isolated from an avian septicemic Escherichia coli (APEC) strain possessing genes related to the adhesion and invasion capacities of in vitro-cultured cells was sequenced. The results demonstrated that the 43-MDa plasmid harbors bacterial pathogenicity-related sequences which probably allow the wild-type pathogenic strain to adhere to and ... more

E.G. Stehling; T.A. Campos; V. Azevedo; M. Brocchi; W.D. Silveira
12/27/2005
Escherichia coli; IHF protein; Weight matrix

We examined general aspects of the DNA-protein interaction between the integration host factor (IHF) global regulator and its regulatory binding sites in the Escherichia coli K12 genome. Two models were developed with distinct weight matrices for the regulatory binding sites recognized by IHF. Using these matrices we performed a genome scale scan and built a set of ... more

Tdos Santos; P.Sérgio Rodrigues
07/19/2011
Aminoglycoside resistance; Escherichia coli; Homologous recombination gene (recA); Neomycin phosphotransferase gene (nptII)

In an attempt to clone the ORF of the nptII gene of Escherichia coli K12 (ATCC 10798), two degenerate primers were designed based on the nptII sequence of its Tn5 transposon. The nptII ORF was placed under the control of the E. coli hybrid trc promoter, in the pKK388-1 vector, transformed into E. coli DH5a Δrec ... more

S. Ghanem
08/24/2012
E3 ligase; Escherichia coli; Maltose; Proteosome; Ubiquitin

SINAT5 is a plant E3 ligase that regulates auxin signaling and root morphogenesis by ubiquitination of the NAC1 protein. Consequently, it may be a putative regulator of aspects of plant development cycles that are controlled by auxin. Efficient production, purification and correctly folded form of this protein are important requirements for functional studies. We produced and ... more

A. Gholizadeh
01/29/2014
Commensal; Escherichia coli; MALDI-TOF; Mass fingerprint; Pathotypes

The mass profiles of cell-free extracts of 180 commensal and pathogenic strains of Escherichia coli were determined by MALDI-TOF mass spectrometry (MS). While some peaks were highly conserved in all E. coli, several peaks occurred only in some strains, showing heterogeneity among them. We did not detect strain-specific peaks for any of the E. coli categories ... more

C.B. Dallagassa; L.F. Huergo; M.I. Stets; F.O. Pedrosa; E.M. Souza; L.M. Cruz; F.E.A. Assis; S. Wolf; W. Volanski; G. Picheth; C.P. Pigatto-Denardi; S.M.S.S. Farah; C.M.T. Fadel-Picheth