Research Report

Related GMR Articles

Canidae; Chrysocyon brachyurus; Cross-amplification; Domestic dog; Microsatellite; Population genetics

The maned wolf (Chrysocyon brachyurus) is the largest South American canid. Habitat loss and fragmentation, due to agricultural expansion and predatory hunting, are the main threats to this species. It is included in the official list of threatened wildlife species in Brazil, and is also protected by IUCN and CITES. Highly variable genetic markers such as microsatellites have ... more

D.C. Salim; A.A. Akimoto; C.B. Carvalho; S.F. Oliveira; C.K. Grisolia; J.R. Moreira; M.N. Klautau-Guimarães
Genetic distance; Genome; Microsatellite; Molecular markers; Population genetics; Triticum aestivum

We investigated the genetic diversity of 63 wheat genotypes, composed of 48 accessions and 15 varieties, using 56 polymorphic simple sequence repeat primers. One hundred and eighty-six loci were found, with a mean of 131.26 alleles per locus. Cluster analysis based on microsatellite allelic diversity discrimi­nated the accessions and varieties into different clusters; genetic di­versity ... more

S. Ijaz; I.A. Khan
Brazilian Atlantic forest; conservation genetics; Microsatellite; Population genetics; Tropical tree species

Cariniana legalis is one of the largest tropical trees with a wide distribution in the Brazilian Atlantic rainforest. We investigated the Mendelian inheritance, genetic linkage, and genotypic disequilibrium at seven microsatellite loci specifically isolated for C. legalis, and at two previously developed heterologous microsatellite loci. Forty to 100 open-pollinated ... more

E.V. Tambarussi; R. Vencovsky; M.L.M. Freitas; A.M. Sebbenn
Brassicaceae; Microsatellite; Population genetics; Wild Brassica juncea

Wild Brassica juncea is a widespread weed in China with increasingly great impact on the yield of many crops. This study aimed to develop microsatellite markers for assessing the genetic diversity and population genetic structure of B. juncea, and to provide basic information for biological and chemical control of the weed. The compound microsatellite marker technique ... more

Y. Li; X.Q. Sun; Q.Q. Yan; J.L. Guo; S. Qiang; X.L. Song; M.M. Li
Gene flow; Genetic diversity; Lepidoptera evolution; Management units; Microsatellite; Population genetics

We present polymorphic microsatellite markers isolated for genetic studies of the sugarcane borer, Diatraea saccharalis (Fabricius). We isolated 16 microsatellite loci through an enriched genomic library protocol. After characterization, 12 markers showed polymorphic information expressed in the observed number of alleles (ranging from 2 to 7; 5 on average) and in the ... more

V.A.C. Pavinato; K.L. Silva-Brandão; M. Monteiro; M.I. Zucchi; J.B. Pinheiro; F.L.F. Dias; C. Omoto
Amorphophallus konjac; Microsatellite; Population genetics; Tuber crop

Amorphophallus konjac is an herbaceous tuber crop with tremendous potential for commercial development. We report the development of microsatellite primers for this important crop species. Thirteen polymorphic microsatellite markers were developed and tested in two populations of A. konjac from the Wuling Mountain Region (WL population) and the Yunnan Province ( ... more

C. Pan; Y.N. You; Y. Diao; Z.L. Hu; J.M. Chen
Microsatellite; Molecular marker; Paris polyphylla var. chinensis; Population genetics

Paris polyphylla var. chinensis is a perennial herb with medicinal properties that is widely used in traditional Chinese medicine. However, this plant has been on the edge of extinction during the last few decades because of excessive deforestation based on the intense ethnopharmaceutical interest. We isolated 12 microsatellite loci from a (CT)n-enriched ... more

J.Y. Zheng; H. Wang; X.X. Chen; P. Wang; P. Gao; X.N. Li; G.P. Zhu
Invasive plant; Microsatellite; Population genetics; Solidago canadensis

Solidago canadensis, a clonal herb originally from North America (common name: Canada goldenrod), is an invasive species in many countries. We developed microsatellite primers for this species. Eleven polymorphic loci were generated and primers were designed. Polymorphism of these 11 loci was assessed in 35 plants from two populations (Wuhan and Shanghai) in China. The number of ... more

S.Y. Zhao; S.G. Sun; Y.H. Guo; J.M. Chen; Q.F. Wang
Arnica; Medicinal plant; Microsatellite; Population genetics

Lychnophora ericoides and Lychnophora pinaster are species used in popular medicine as analgesic or anti-inflammatory agents to treat contusions, rheumatism, and insect bites. In this study, 21 simple sequence repeat loci of L. ericoides were developed and transferred to L. pinaster. Three populations of L. ericoides and 2 populations of L ... more

M.A.R. Vieira; M.O.M. Marques; L.L. Haber; B.B.Z. Vigna; M.M. Bajay; J.B. Pinheiro; A.P. Souza; J. Semir; M.I. Zucchi
Chinese muntjac; Microsatellite; Muntiacus reevesi; Polymorphism; Population genetics

Eight polymorphic microsatellite markers for Muntiacus reevesi were identified and characterized in this study. The number of alleles per locus ranged from 3 to 10 across 24-48 samples. The loci showed expected and observed heterozygosities of 0.577-0.876 and 0.387-0.933, respectively, with an average polymorphic information content value of 0.682. These markers should be a ... more

H. Wang; X. Luo; W.B. Shi; B.W. Zhang