Research Article

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3-1; 4-glucanase; Antifungal activity; Bacillus subtilis; Heterologous expression; Sclerotinia sclerotiorum; β-1

An antifungal protein exhibiting a high activity against Sclero­tinia sclerotiorum in vivo was purified by ammonium sulfate precipitation, hydrophobic chromatography, and gel filtration chromatography from the culture filtrate of the endophytic Bacillus subtilis strain Em7. The protein was characterized as a β-1,3-1,4-glucanase according to amino acid analysis, and showed ... more

N.N. Wang; X.N. Gao; X. Yan; Z.P. Li; Z.S. Kang; L.L. Huang; Q.M. Han
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Bacillus subtilis; BIOLOG ECO microplate analysis; Functional diversity; Glyphosate degradation; Microbial communities; Soil bioremediation

Glyphosate and glyphosate-containing herbicides have an adverse effect on mammals, humans, and soil microbial ecosystems. Therefore, it is important to develop methods for enhancing glyphosate degradation in soil through bioremediation. We investigated the potential of glyphosate degradation and bioremediation in soil by Bacillus subtilis Bs-15. Bs-15 grew well at high ... more

X.M. Yu; T. Yu; G.H. Yin; Q.L. Dong; M. An; H.R. Wang; C.X. Ai
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Bacillus subtilis; Mutagenesis; Purification; Serine protease; Strain improvement

The feather is a valuable by-product with a huge annual yield produced by the poultry industry. Degradation of feathers by microorganisms is a prerequisite to utilize this insoluble protein resource. To improve the degrading efficiency of feathers, mutagenesis of the bacterium Bacillus subtilis S1-4 was performed. By combining ultraviolet irradiation and N-methyl-N’-nitro-N- ... more

X.C. Wang; H.Y. Zhao; G. Liu; X.J. Cheng; H. Feng; X.C. Wang; H.Y. Zhao; G. Liu; X.J. Cheng; H. Feng
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28S rDNA; Colletotrichum; ITS; Molecular phylogeny; β-tubulin

The genus Colletotrichum contains many important plant pathogens mainly distributed in tropical areas. Previous studies that utilized morphology or single genes have failed to resolve the phylogenetic relationships among the species. In this study, sequences of β-tubulin, 28S ribosomal DNA, and ITS region from nine species were analyzed separately and combined to establish a fast ... more

H. Chen; Y. Ma; W.F. Zhang; T. Ma; H.X. Wu
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Antimicrobial activity; Colletotrichum; Crude extracts; Endophyte; Mycovirus; Phytopathogen

Fungi belonging to the Colletotrichum genus can be categorized as endophytic or phytopathogenic. These fungi can be infected by viruses, termed mycoviruses, which are know to promote hypovirulence in infected fungi. However, there are few studies that have described mycoviral infections of endophytes. The production of secondary metabolites by endophytes with antimicrobial ... more

P. Rosseto; A.T. Costa; J.C. Polonio; A.A. da Silva; J.A. Pamphile; J.L. Azevedo; P. Rosseto; A.T. Costa; J.C. Polonio; A.A. da Silva; J.A. Pamphile; J.L. Azevedo
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Chili pepper (Capsicum chinense) is a great economic important culture on the State of Amazonas, and it represents, approximately, a production of 1.9 thousand tons per year. It is one of the hosts of Colletotrichum genus in the North region of Brazil. The aim of the study was to differentiate and to identify isolates of Colletotrichum collected from C. chinense in Amazon. Molecular ... more

C.V.S. de Oliveira; K.S. Matos; D.M.C. de Albuquerque; R.E. Hanada; G.F. da Silva
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Capsicum; Colletotrichum; Genotype; Plant breeding; Plant Immunity; Selection, Genetic; Selective breeding

Anthracnose is among the major diseases of the Capsicum culture. It is caused by different species of the genus Colletotrichum, which may result in major damages to the cultivation of this genus. Studies aiming to search for cultivars resistant to diseases are essential to reduce financial and agricultural losses. The objective of this study was to evaluate the correlation between the ... more

A.C. Maracahipes; J.W.S. Correa; P.E. Teodoro; K.L. Araújo; M.A.A. Barelli; L.G. Neves
08/17/2017
Capsicum; Colletotrichum; Genotype; Plant breeding; Plant Immunity; Selection, Genetic; Selective breeding

Anthracnose is among the major diseases of the Capsicum culture. It is caused by different species of the genus Colletotrichum, which may result in major damages to the cultivation of this genus. Studies aiming to search for cultivars resistant to diseases are essential to reduce financial and agricultural losses. The objective of this study was to evaluate the correlation between the ... more

A.C. Maracahipes; J.W.S. Correa; P.E. Teodoro; K.L. Araújo; M.A.A. Barelli; L.G. Neves
08/17/2017
Capsicum; Colletotrichum; Genotype; Plant breeding; Plant Immunity; Selection, Genetic; Selective breeding

Anthracnose is among the major diseases of the Capsicum culture. It is caused by different species of the genus Colletotrichum, which may result in major damages to the cultivation of this genus. Studies aiming to search for cultivars resistant to diseases are essential to reduce financial and agricultural losses. The objective of this study was to evaluate the correlation between the ... more

A.C. Maracahipes; J.W.S. Correa; P.E. Teodoro; K.L. Araújo; M.A.A. Barelli; L.G. Neves
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Alcohol Oxidoreductases; Anthocyanins; Cloning, Molecular; Flavonols; Fruit; Gene Expression Regulation, Plant; Glucosyltransferases; Organ Specificity; Plant Proteins; Transcription factors; Vitis

MYBA2 transcription factor (Myb-related gene) affects the coloring in grapevine berry and plays an important role in the biosynthesis of anthocyanin. The MYBA2 gene was cloned from Vitis vinifera L. cv. Cabernet Sauvignon and polyclonal antibodies for VvmybA2 were prepared. The VvmybA2 gene expression patterns were observed in seven tissues (the leaf, stem, flower, bud, root, berry, and ... more

T.Q. Niu; Z.D. Gao; P.F. Zhang; X.J. Zhang; M.Y. Gao; W. Ji; W.X. Fan; P.F. Wen

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