Research Article

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2017 May 10
Adaptation, Physiological; Droughts; Genotype; Germination; Glycine max; Osmotic pressure; Plant breeding; Selection, Genetic

Water deficit is the main reason for instability in the context of soybean culture. The development of strategies for the selection of more tolerant genotypes is necessary. These strategies include the use of polyethylene glycol 6000 solutions (PEG-6000) for conducting the germination test under conditions of water restriction. Thus, the objective of this study was to determine the osmotic ... more

S.A.G. Dantas; F.C.S. Silva; L.J. Silva; F.L. Silva
12/02/2016
Deoxyribonucleases; DNA; Gene expression; Plant Leaves; RNA, Plant; Saccharum

The success of gene expression studies, protein synthesis, and construction of cDNA libraries directly depends on the purity and integrity of the RNA used in these tests, as even minor amounts of contaminant DNA (<1%) can produce a false positive amplification signal in quantitative real-time PCR. For RNA contaminated with genomic DNA, an essential step in the studies on gene expression is ... more

J.A. Santos; G.A. Luz; K.P. Oliveira; L.F. Oliveira; A.S.Andrade Júnior; S.E.S. Valente; P.S.C. Lima
08/17/2017
Alcohol Dehydrogenase; Catalase; Esterases; Germination; Glutamate Dehydrogenase; Isocitrate lyase; Malate dehydrogenase; Osmotic pressure; Plant Proteins; seeds; Sesamum; Superoxide dismutase

With the imminence of global climate changes that affect the temperature and the rainfall uniformity, it is growing the concern about the adaptation of crops to the water deficit. Thus, the objective of this study was to evaluate alterations in physiological and enzymatic mechanisms during the germination process of sesame seeds under different water availability. To simulate the water ... more

R.M.O. Pires; M.A.B. Àvila; D.G. Leite; H.O. Santos; G.A. Souza; E.V.R. Von Pinho
12/23/2016
Fruit; Gene expression profiling; Gene Expression Regulation, Plant; Mutation; Prunus persica; Transcriptome

Fruit ripening is a complex developmental process, the details of which remain largely unknown in fleshy fruits. In this paper, the fruit flesh of two peach varieties, "Zhongyou9" (a nectarine; Prunus persica L. Batsch) and its mutant "Hongyu", was analyzed by RNA-seq technology during two stages of ripening at 20-day intervals. One hundred and eighty significant upregulated and two hundred ... more

H.F. Pan; Y. Sheng; Z.H. Gao; H.L. Chen; Y.J. Qi; X.K. Yi; G.H. Qin; J.Y. Zhang
3/16/2017
Adaptation, Physiological; Copper; Gene Expression Regulation, Plant; Metallothionein; Plant Leaves; Plant Proteins; Prosopis; Time Factors

For a better understanding of the strategies that are used by Prosopis glandulosa in heavy metal tolerance, the present study evaluated the gene expression of three metallothioneins (MTs; PgMt2-1, PgMt2, and PgMt3) in plants exposed to sub-lethal concentrations of copper. The PgMt2-1, PgMt2, and PgMt3 sequences were homologous to the MT type 2 (isoform 1), Mt2, and Mt3 sequences of other plant ... more

C. Michel-Lopez; O. Zapata-Pérez; D. González-Mendoza; O. Grimaldo-Juarez; C. Ceceña-Duran; O. Tzintzun-Camacho
3/08/2017
Adaptation, Physiological; Arachis; Calcium-Binding Proteins; Droughts; Plant Leaves; Plant Proteins; Plants, Genetically Modified; rab GTP-Binding Proteins; Salinity; Salt-Tolerant Plants; Signal transduction; Stress, Physiological; Transcription factors

The Rab protein family is the largest family of the small GTP-binding proteins. Among them, the RabG genes are known to be responsive to abiotic stresses, but the molecular mechanisms of the stress responses mediated by RabG genes in plants is poorly understood. To investigate the molecular mechanism of AhRabG gene in peanut, transgenic plants overexpressing the AhRabG gene (S6) with ... more

J.M. Sui; G. Li; G.X. Chen; M.Y. Yu; S.T. Ding; J.S. Wang; L.X. Qiao
2017 Mar 30
Abscisic acid; Adaptation, Physiological; Citrus; Dioxygenases; Droughts; Nicotiana; Plant Leaves; Plant Proteins; Plants, Genetically Modified; Stress, Physiological

9-cis-epoxycarotenoid dioxygenase (NCED) encodes a key enzyme in abscisic acid (ABA) biosynthesis. Little is known regarding the regulation of stress response by NCEDs at physiological levels. In the present study, we generated transgenic tobacco overexpressing an NCED3 ortholog from citrus (CsNCED3) and investigated its relevance in the regulation of drought stress tolerance. Wild-type (WT) ... more

A.M. Pedrosa; L.C. Cidade; C.P.S. Martins; A.F. Macedo; D.M. Neves; F.P. Gomes; E.I.S. Floh; M.G.C. Costa
10/04/2007
Defense; Expression profile; Kinase; NBS-LRR; Pathogens; Saccharum

Resistance genes (R-genes) are responsible for the first interaction of the plant with pathogens being responsible for the activation (or not) of the defense response. Despite their importance and abundance, no tools for their automatic annotation are available yet. The present study analyzed R-genes in the sugarcane expressed sequence tags database which includes 26 ... more

A.C. Wanderley-Nogueira; N.M. Soares-Cavalcanti; D.A.L. Morais; L.C. Belarmino; A. Barbosa-Silva; A.M. Benko-Iseppon
06/01/2010
DNA marker; Fingerprinting; Genetic distance; Saccharum; SSR

Forty genotypes (clones) of sugarcane, including elite lines, commercial cultivars of Saccharum officinarum and clones of S. barberi were fingerprinted with 50 SSR markers using a PCR-based marker assay. Nei’s genetic distances for SSR data were determined and relationships between accessions were portrayed graphically in the form of a dendrogram. Genetic distance values ... more

S. Nawaz; F.A. Khan; S. Tabasum; M.Z. Iqbal; A. Saeed
03/12/2014
Genetic improvement; Multi-diverse analysis; Saccharum

This study evaluated different strategies to select sugar cane families and obtain clones adapted to the conditions of the Brazilian savannah. Specifically, 7 experiments were conducted, with 10 full sib families, and 2 witnesses in common to all experiments, in each experiment. The plants were grown in random blocks, with witnesses in common (incomplete blocks), and 6 repetitions of each ... more

M.H.P. Barbosa; A. Ferreira; L.A. Peixoto; M.D.V. Resende; M. Nascimento; F.F. Silva

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