Research Article

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09/27/2017
Arachis; Genotype; Plant Leaves; Plant Proteins; Resveratrol; RNA, Messenger; Stilbenes; Tetraploidy; Transcriptional activation; Ultraviolet Rays

Resveratrol is an antioxidant that is a promising antitumoral, cardioprotective and neuroprotective agent. It has been found in a restricted number of plants including peanut (Arachis hypogaea L.) and its wild relatives. The objective of this study was to understand the relationship between resveratrol content and the expression of putative resveratrol synthase genes in four Arachis genotypes ... more

P.A.S.V. Carvalho; A.C. Brasileiro; S. Leal-Bertioli; D.J. Bertioli; J.P. Silva; T.S. Agostini-Costa; M.A. Gimenes
12/19/2016
3' Untranslated Regions; Animals; Cell differentiation; Cell proliferation; Cells, Cultured; Down-regulation; Gene Expression Regulation, Developmental; Microfilament Proteins; MicroRNAs; Muscle development; Muscle Proteins; Myoblasts; Swine

MicroRNAs (miRNAs) play important roles in the development and biochemical functions of skeletal muscles. However, targeting of miRNAs to structural genes involved in Z-discs have not been investigated. Here, we describe a highly expressed miRNA, miR-432, in pig embryonic skeletal muscle, which appeared to target myozenin1 (MYOZ1), a protein involved in the muscular sarcomere microstructure. ... more

R.M. Ren; H. Liu; S.H. Zhao; J.H. Cao
3/16/2017
Animals; Animals, Newborn; Cation Transport Proteins; Gene Expression Regulation, Developmental; Immunity, Innate; Sus scrofa; Swine; Tissue distribution; Weaning

Natural resistance-associated macrophage protein gene 1 (Nramp1) plays an important role in the innate immune response of swine, and is believed to influence disease resistance. In this study, a real-time quantitative polymerase chain reaction technique was used to investigate Nramp1 expression in 12 different tissues in newborn and 7-, 14-, 21-, 28-, and 35-day-old Meishan piglets. Results ... more

C.H. Dai; J.Y. Wu; C.X. Zhao; L.H. Yu; W.B. Bao; S.L. Wu
4/13/2017
Animals; Animals, Inbred Strains; Ear; Extracellular Matrix Proteins; Gene expression profiling; Genome-wide association study; Intercellular Signaling Peptides and Proteins; Methionine Sulfoxide Reductases; Quantitative trait loci; RNA, Messenger; Swine

Porcine ear size is an important characteristic for distinguishing among pig breeds. In a previous genome-wide association study of porcine ear size, LEM domain-containing 3 (LEMD3), methionine sulfoxide reductase B3 (MSRB3), high mobility group AT-hook 2 (HMGA2), and Wnt inhibitory factor 1 (WIF1) were implicated as important candidate genes for ear size. This study investigated the ... more

L.C. Zhang; J. Liang; L. Pu; Y.B. Zhang; L.G. Wang; X. Liu; H. Yan; L.X. Wang
05/25/2017
Animals; Apoptosis Regulatory Proteins; meat; Polymorphism, Single Nucleotide; Quantitative Trait, Heritable; RNA, Messenger; Swine

Cell death-inducing DFF45-like effector (CIDE) B is a member of the CIDE family of apoptosis-inducing factors. In the present study, we detected a single nucleotide polymorphism (SNP), c.414G>A, which corresponds to the synonymous SNP 414Arg, in CIDE-B in the Berkshire pigs. We also analyzed the relationships between the CIDE-B SNP and various meat quality traits. The SNP was significantly ... more

G.E. Yu; S. Kwon; J.H. Hwang; S.M. An; D.H. Park; D.G. Kang; T.W. Kim; I.S. Kim; H.C. Park; J. Ha; C.W. Kim
09/21/2017
Animals; Diacylglycerol O-Acyltransferase; Genotype; Growth hormone; Leptin; Polymorphism, Single Nucleotide; Receptors, Leptin; Selective breeding; Swine

Molecular markers are tools used to improve genetic gains. The objective of this study was to analyze the security of alleles of molecular marker genes for characteristics of economic interest in a pure population of pigs. After the extraction of DNA from the hair of 272 Large White matrices, the allele and genotype frequency of single nucleotide polymorphism was performed using the ARMS-PCR ... more

I.M. Ferreira; G.S. Vieira; T.F. Braga; T.C.F. Silva; M.M. Franco; R.C. Antunes
4/05/2017
Chromosomes, Plant; DNA methylation; DNA, Plant; Epigenesis, Genetic; Evolution, Molecular; Genetic variation; Genome, Plant; Microsatellite Repeats; Polyploidy; Random Amplified Polymorphic DNA Technique; Triticum

We used the conventional and methylation-sensitive randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) analyses to assess genome-wide changes and explore the relationships between genetic and epigenetic variations among individuals of a newly synthesized allohexaploid wheat line whose genomic constitution is identical to that of the natural common wheat, compared ... more

T. Qiu; Y.Z. Dong; X.M. Yu; N. Zhao; Y.F. Yang
5/04/2017
DNA methylation; Nucleic Acid Amplification Techniques; Polymorphism, Genetic; Saccharum

Micropropagation is an important tool for large-scale multiplication of plant superior genotypes. However, somaclonal variation is one of the drawbacks of this process. Changes in DNA methylation have been widely reported as one of the main causes of somaclonal variations in plants. In order to investigate the occurrence of changes in the methylation pattern of sugarcane somaclonal variants, ... more

J.H.M.B. Francischini; E.L. Kemper; J.B. Costa; J.R.V. Manechini; L.R. Pinto
06/29/2017
Chromosomes, Plant; DNA methylation; Genome, Plant; Genome-wide association study; Gossypium; Salinity; Stress, Physiological

Cytosine DNA methylation is a significant form of DNA modification closely associated with gene expression in eukaryotes, fungi, animals, and plants. Although the reference genomes of cotton (Gossypium hirsutum L.) have been publically available, the salinity-stress-induced DNA methylome alterations in cotton are not well understood. Here, we constructed a map of genome-wide DNA methylation ... more

X.K. Lu; N. Shu; J.J. Wang; X.G. Chen; D.L. Wang; S. Wang; W.L. Fan; X.N. Guo; L.X. Guo; W.W. Ye
12/02/2016
Animals; Brazil; Breeding; Cattle; Gene frequency; Genetic markers; Leptin; Polymorphism, Single Nucleotide; Promoter Regions, Genetic; Red Meat; Sequence Analysis, DNA

Brazil is the world's largest producer of beef cattle; however, the quality of its herds needs to be improved. The use of molecular markers as auxiliary tools in selecting animals for reproduction with high pattern for beef production would significantly improve the quality of the final beef product in Brazil. The leptin gene has been demonstrated to be an excellent candidate gene for bovine ... more

R.C. Guimarães; J.S.N. Azevedo; S.C. Corrêa; J.E.G. Campelo; E.M. Barbosa; E.C. Gonçalves; S. Filho

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