Research Article

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The current study aimed to investigate the coding sequence, polymorphisms, and expression of the RERG gene in indigenous Chinese goats. cDNA of RERG, obtained through reverse transcription PCR was analyzed using bioinformatic techniques. Polymorphisms in the exon regions of the RERG gene were identified and their associations with growth traits in three varieties of ... more

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Coffee seeds begin to develop shortly after fertilization and can take 6 to 8 months to complete their formation, a period during which all the characteristics of the mature seed are determined, directly influencing physiological quality. However, little is known about the molecular mechanisms that act during coffee seed maturation. The objective of the current study was to analyze ... more

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A. Ud-Din; M. Rauf; S. Ghafoor; M.N.K. Khattak; M.W. Hameed; H. Shah; S. Jan; K. Muhammad; A. Rehman; Inamullah; A. Ud-Din; M. Rauf; S. Ghafoor; M.N.K. Khattak; M.W. Hameed; H. Shah; S. Jan; K. Muhammad; A. Rehman; Inamullah; A. Ud-Din; M. Rauf; S. Ghafoor; M.N.K. Khattak; M.W. Hameed; H. Shah; S. Jan; K. Muhammad; A. Rehman; Inamullah
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Abscisic acid; Coffea arabica; Gibberellic acid; qRT-PCR

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The aim of this study was to clone the isoflavone synthase (IFS) gene and establish the recombinant Minshan Trifolium pratense. The IFS gene was cloned from the callus of Minshan T. pratense using reverse transcription-polymerase chain reaction. The plant expression vector pRI101-AN-IFS was constructed and introduced into Agrobacterium tumefaciens strain LBA4404, and ... more

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Primers were designed according to the Cu/Zn-SOD gene sequences of cloned Cucurbits plants (cucumbers and watermelons) available in NCBI. Total RNA from Hami melon pulp was used as a template. Following RT-PCR amplification, a 403-bp fragment of the Hami melon Cu/Zn-SOD gene was obtained. According to alignment in BLAST and phylogenetic tree analysis, the cloned gene fragment was ... more

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