Methodology

Related GMR Articles

09/29/2009
Cattle; Effective population size; Inbreeding; Pigs; Sheep; Software

Genetic variation provides a basis upon which populations can be genetically improved. Management of animal genetic resources in order to minimize loss of genetic diversity both within and across breeds has recently received attention at different levels, e.g., breed, national and international levels. A major need for sustainable improvement and conservation programs is accurate ... more

E. Groeneveld; Bv.d. Westhuizen; A. Maiwashe; F. Voordewind; J.B.S. Ferraz
05/03/2011
Adipocyte fatty acid-binding protein; Gene expression; Longissimus dorsi; meat quality; Pigs; Polymorphism

Recent attention in pig breeding programs has focused on the improvement of pork quality in response to increasing consumer demands. Compared to the fatty-type Northeastern Indigenous (Chinese) breed of pigs, the lean-type Large White has lower intramuscular fat and inferior eating quality from the perspective of the Chinese consumer. In order to investigate the molecular basis of ... more

Y. Gao; Y.H. Zhang; H. Jiang; S.Q. Xiao; S. Wang; Q. Ma; G.J. Sun; F.J. Li; Q. Deng; L.S. Dai; Z.H. Zhao; X.S. Cui; S.M. Zhang; D.F. Liu; J.B. Zhang
10/18/2013
Free amino acids; Gene expression; performance; Pigs; Protein-bound

Free amino acids (AA) appear to be absorbed faster than protein-bound AA (PB-AA). We conducted an experiment to assess the effect of feeding pigs with a partially free (F-AA) or totally PB-AA diet on expression of selected genes and performance of pigs. The expression of cationic AA transporters b0,+ and CAT-1 in intestinal mucosa, liver, and longissimus (LM) and ... more

A. Morales; F. Grageola; H. García; A. Araiza; R.T. Zijlstra; M. Cervantes
04/16/2014
ANGPTL4; Association analysis; Differential expression; Intramuscular fat; Pigs

In a previous study, we investigated differences in gene expression in backfat between Meishan and Large White pigs and their F1 hybrids, Large White x Meishan, and Meishan x Large White pigs. One potential differentially expressed sequence tag from the mRNA differential display was a homolog of the human angiopoietin-like 4 (ANGPTL4) gene, which encodes a protein that ... more

Z.Q. Ren; W.J. Wu; W.H. Liu; R. Zheng; J.L. Li; B. Zuo; D.Q. Xu; F.E. Li; M.G. Lei; D.B. Ni; Y.Z. Xiong
03/12/2014
AS-PCR (allele-specific PCR); IGFBP-5 gene; Pigs; SNP

The genetic resources and the mechanism of miniaturization in the Tibet Mini-pig have not been comprehensively studied. Polymorphisms in genes related to the insulin-like growth factor (IGF) axis have been investigated for years, but few on the polymorphism of IGF-binding protein-5 (IGFBP-5) in the Tibetan pig. In this study, allele-specific polymerase chain reaction (AS-PCR) was used to ... more

A.D. Chen; L.L. Hao; X.B. Fang; K. Lu; S.C. Liu; Y.L. Zhang
03/31/2015
Hepatitis E virus; Liver; Pigs; Reverse transcription-polymerase chain reaction; Zoonosis

Hepatitis E is a form of endemic acute hepatitis found in humans in many countries worldwide and is caused by the hepatitis E Virus (HEV). Detection of HEV in pigs indicates that they may be carriers, possibly through zoonosis. The prevalence of HEV in pigs in Colombia is unknown. Studies in the US found that 11% of pig livers sold in grocery stores are contaminated with HEV. It is also ... more

C. Gutiérrez-Vergara; J. Quintero; J.F. Duarte; J.P. Suescún; A. López-Herrera
03/06/2015
Gene expression; Leu:Lys ratio; Pigs; Serum amino acids

We examined 96 pigs (28.1 ± 0.83 kg) to analyze the effect of Leu:Lys ratios on expression of the cationic amino acid transporters b0,+ and CAT-1 in the jejunum and liver as well as myosin expression in 2 muscles to estimate the optimum standardized ileal digestible (SID) Leu:Lys ratio for growth rate and efficiency. A wheat-and wheat bran-based diets were formulated to meet ... more

H. García; A. Morales; A. Araiza; J.K. Htoo; M. Cervantes