Research Article

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Barker frog; Microsatellite; Mitochondrial DNA; Physalaemus; Population structure

The barker frog Physalaemus cuvieri is widely distributed in South America and is found in all regions of Brazil. Significant intraspecific morphological variation in this species has been reported. To determine the genetic structure of the natural Brazilian populations of P. cuvieri, 10 different populations geographically separated by 99.41 to 2936.75 km were evaluated ... more

M. Conte; C.P. Targueta; M.I. Zucchi; A.P. Souza; S.M. Recco-Pimentel
Melipona mondury; Melipona rufiventris; Meliponini; Mitochondrial DNA; Polymorphic site; RFLP

Within the Meliponini, a widely distributed group of stingless bees, Melipona rufiventris has been considered as a single, cohesive species. Recently, analysis of morphological characters led to the splitting of this species into two species, M. mondury and M. rufiventris. The former occurs in the Atlantic Rain Forest ranging from Santa Catarina to Bahia ... more

G.S. Barni; R. Strapazzon; J.C.V. Guerra; G. Moretto
Conservation; Endangered species; Mitochondrial DNA; Oryx; Phylogenetic relationship

The present study reports the application of mitochondrial markers for the molecular phylogeny of Oryx species, including the Arabian oryx (AO), scimitar-horned oryx (SHO) and plains oryx (PO), using the Addax as an outgroup. Sequences of three molecular markers, 16S rRNA, cytochrome b and a control region, for the above four taxa were aligned and the topologies of respective ... more

H.A. Khan; I.A. Arif; A.A.Al Homaidan; A.H.Al Farhan
Mitochondrial DNA; Polymorphism; Sea turtles; Single strand conformation polymorphism

Marine turtles are increasingly being threatened worldwide by anthropogenic activities. Better understanding of their life cycle, behavior and population structure is imperative for the design of adequate conservation strategies. The mtDNA control region is a fast-evolving matrilineal marker that has been employed in the study of marine turtle populations. We developed and tested a simple ... more

E.C. Reis; R.M. Albano; A.C.V. Bondioli; L.S. Soares; G. Lôbo-Hajdu; G. Lôbo-Hajdu
Endangered animals; Mitochondrial DNA; Oryx species; Phylogeny; rRNA genes

We conducted a comparative evaluation of 12S rRNA and 16S rRNA genes of the mitochondrial genome for molecular differentiation among three oryx species (Oryx leucoryx, Oryx dammah and Oryx gazella) with respect to two closely related outgroups, addax and roan. Our findings showed the failure of 12S rRNA gene to differentiate between the genus Oryx and addax ... more

H.A. Khan; I.A. Arif; A.H.Al Farhan; A.A.Al Homaidan
Bos indicus; Bos taurus; Guzerat; Mitochondrial DNA; production; Reproduction

The observation of bovine mitochondrial DNA (mtDNA) polymorphisms allows the separation of American zebu cattle, according to its maternal lineage ancestry, into two groups: one with Bos indicus mtDNA and other with Bos taurus mtDNA. The aim of the present study was to determine the productive and reproductive differences between these two groups, in a Guzerat dairy herd. The ... more

J.C.C. Paneto; J.B.S. Ferraz; J.C.C. Balieiro; J.F.F. Bittar; M.B.D. Ferreira; M.B. Leite; G.K.F. Merighe; F.V. Meirelles
Cytochrome C oxidase 1; Mitochondrial DNA; Molecular phylogeny

Morphology and biogeography are widely used in animal taxonomy. Recent study has suggested that a DNA-based identification system, using a 648-bp portion of the mitochondrial gene cytochrome oxidase subunit 1 (CO1), also known as the barcoding gene, can aid in the resolution of inferences concerning phylogenetic relationships and for identification of species. However, ... more

D.B. Yu; R. Chen; H.A. Kaleri; B.C. Jiang; H.X. Xu; W.X. Du
Hucul horse; Mitochondrial DNA; Phylogeny; Primitive

The existence of the Hucul horse on Romanian territory has been documented from the very distant past; today Hucul is a unique breed that is part of the FAO Program for the Preservation of Animal Genetic Resources. We compared Hucul with several primitive European and Asiatic breeds in order to elucidate the origin of these horses. We analyzed a 683-bp mitochondrial DNA (mtDNA) D-loop ... more

S.E. Georgescu; M.A. Manea; A. Dudu; M. Costache
Artificial reproduction; Black rockfish; Genetic variability; Microsatellite loci; Mitochondrial DNA; Sebastes inermis

The black rockfish, Sebastes inermis (Sebastidae), is an important commercial fishery resource in Korea. As a preliminary investigation into the effect of artificial reproduction in a hatchery facility, the genetic divergence between parent and offspring populations of black rockfish was accessed using 10 polymorphic nuclear microsatellite DNA loci and a mitochondrial (mt) ... more

H.S. An; J.W. Lee; J.Y. Park; J.I. Myeong; C.M. An
Malaria; Mitochondrial DNA; Plasmodium falciparum; Plasmodium vivax; Real-time PCR

The need for a more sensitive and time-efficient assay for malaria has led to the development of molecular assays involving real-time PCR (qPCR), a procedure that has the potential to detect low levels of parasitemia, identify mixed infections, and allow for precise differentiation of species via melting curve analysis or TaqMan fluorescence-labeled probes. Since the first study published ... more

C.R.T. Souza; T.A.A. Carvalho; R.C.G. Amaral; L.S. Cunha; M.G. Cunha; J.F. Guerreiro