Research Article

Related GMR Articles

09/30/2003
Bovine; Comparative map; FISH; Gene assignment

Seven genes were assigned by molecular cytogenetic methods to bovine chromosome 5. To accomplish this, specific primers were either publicly available or were designed from highly conserved regions of the publicly available mammalian gene sequences. The identity of the amplified segments was verified by sequencing and alignment with the published sequences. The optimized primers that amplified ... more

M. De Donato; D.S. Gallagher; C. Lehn; C. Gill; J.F. Taylor
10/05/2007
FISH; Genomic library; Jackknife estimate; Microsatellites; Piracema species; Prochilodus lineatus

We described five novel microsatellite loci for the piracema fish species Prochilodus lineatus (Characiformes), endemic to South America and of extreme importance to both commercial and artisanal fisheries across its occurrence area. A primary, unenriched genomic library was constructed and radioactively screened for repetitive motifs. Positive clones were automatically ... more

G.M. Yazbeck; E. Kalapothakis
12/08/2011
Chromosome banding; FISH; Karyotype variability; Topotypes

A large number of genera in the tropical fish family Characidae are incertae sedis. Cytogenetic analysis was made of four of these species: Astyanax eigenmanniorum, Deuterodon stigmaturus, Hyphessobrycon luetkenii, and H. anisitsi, collected from various hydrographic basins: hydrographic system from Laguna dos Patos/RS, Tramandaí basin/RS and ... more

M.M. Mendes; R. da Rosa; L. Giuliano-Caetano; A.L. Dias
12/08/2011
Chromatin; DNA methylation; FISH; Micrococcal nuclease; Myostatin

Myostatin is a negative regulator of the growth and development of skeletal muscle mass. In fish, myostatin is expressed in several organs in addition to skeletal muscle. To understand the mechanisms regulating myostatin gene expression in the sea perch, Lateolabrax japonicus, we examined the methylation status of the myostatin gene promoter region in several tissues (liver, eye, kidney, brain ... more

E.M. Abbas; A. Takayanagi; N. Shimizu; M. Kato
10/25/2011
FISH; Genetic conservation; RAPD; Surubim

Steindachneridion melanodermatum is a large Brazilian catfish, highly prized for sport fishing and for its meat. Specimens of this species, both caught in nature from Iguacu River and F1 fish born in captivity, were analyzed with regard to patterns of RAPD molecular markers. Genetic similarity ranged from 0.57 to 0.95; two groups were determined for the wild specimens ... more

D.A. Matoso; M. da Silva; M.C.S. Cortinhas; M.M. Cestari; M.C. de Almeida; M.R. Vicari; R.F. Artoni
08/26/2011
DAZ; FISH; PRINS; Single-copy gene; SRY

In order to analyze male sterility caused by deletion of SRY and DAZ, we examined the accuracy and cost-effectiveness of a modified primed in situ labeling (PRINS) technique for detection of single-copy genes. Peripheral blood samples were collected from 50 healthy men; medium-term cultured lymphocytes from these samples were suspended in fixative solution and then spread on ... more

J.L. Gao; Y. Nie; X.P. Ding
07/19/2011
C-banding; FISH; G-banding; Karyotype; Nucleolar organizer regions; Turtle
C.E.S. Zago
11/03/2010
Cytogenetic; FISH; Passion flower; Pectinex

Cytological preparations for the fluorescent in situ hybridization (FISH) technique require cytoplasm-free metaphases, with well-spread chromosomes, for the localization of DNA sequences and chromosome mapping. We tested various procedures for FISH analysis of Passiflora cacaoensis, P. gardneri and hybrid F1 progeny of P. gardneri x P. gibertii ... more

M.M. Souza; J.D. Urdampilleta; E.R. Forni-Martins
01/04/2013
Breast cancer; Chromosome 17; FISH; Metastasis; TP53 gene

Breast cancer is the second most common origin of brain metastases, after lung cancer, and represents 14-20% of all cases. Abnormalities of chromosome 17 are important molecular genetic events in human breast cancer, and several oncogenes and tumor suppressor genes are located on this chromosome. In about half of all human cancers, the tumor suppressor gene TP53, located at 17p13 ... more

D.S. Vasconcelos; F.P.E. da Silva; L.G. Quintana; N.P. Anselmo; M.A.K. Othman; T. Liehr; E.H.C. de Oliveira
04/25/2013
45S rDNA; FISH; Karyotype; NOR; Silver staining; Smilax

The genus Smilax (Smilacaceae) includes species of medicinal interest; consequently, their identification is important for the control of raw material used in the manufacture of phytotherapeutic products. We investigated the karyotype of Smilax rufescens in order to look for patterns that would be useful for comparative studies of this genus. To accomplish this, we ... more

D. Pizzaia; V.M. Oliveira; A.R. Martins; B. Appezzato-da-Glória; E. Forni-Martins; M.L.R. Aguiar-Perecin

Pages